The Human 5.0 Project

Dihexa Explained (2026): The HGF/c-Met “Synapse Builder,” Animal Data, Cancer Concerns, and What Human Trials Show

Tonight we’re breaking down Dihexa (PNB-0408) — the angiotensin IV analog that gets called “synapse-building on steroids.” People throw around claims like “stronger than BDNF” and “neurogenesis peptide,” but what’s real… and what’s internet mythology?
In this episode, we go raw + evidence-first:
What Dihexa actually is (and what it’s not)
The real mechanism: HGF / c-Met signaling and why that matters for synapses
What animal studies suggest (and where it doesn’t translate cleanly)
Oral activity + BBB penetration claims from early research
Why formulation/solubility is a headache (and why that matters for safety)
The huge asterisk: growth-factor pathway risks and why long-term human safety is still an open question
The clinical reality check: what happened with related pathway candidates like fosgonimeton (ATH-1017)
Disclaimer: This content is educational only and not medical advice. Dihexa is not approved as a therapeutic drug. If you have a history of cancer, active malignancy, unexplained lumps, or you’re skipping screening—don’t gamble with growth-factor biology.
 
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
#Dihexa #PNB0408 #Nootropics #BrainHealth #CognitiveEnhancement #Synaptogenesis #Neuroplasticity #Biohacking #Longevity #HumanPerformance #HGFCMet #AlzheimersResearch #NoFluff #TheHuman50Project #UltimateBiohacker10X

5-Amino-1MQ (5A1MQ) Part 2: Dosing Talk, Biomarkers to Track, and How to Spot Hype vs Real Data (2026)

In Part 2 on 5-Amino-1MQ (5A1MQ), we get practical and responsible: how to think like a researcher, how to interpret viral before/afters without getting played, what biomarkers actually matter, and what “oral dosing” patterns are commonly claimed online as of 2026—with a clear reminder that this is not a standardized, published human dosing regimen.
This episode is NOT medical advice. 5-Amino-1MQ is a research compound and is not FDA-approved to treat or prevent any condition. If you suspect metabolic disease, celiac disease, or you’re on medications, talk to a qualified clinician.
In this episode:
What a smart, ethical research mindset looks like
How to analyze online claims (water weight vs fat loss, confounders, placebo, “without changing anything”)
What to track if human trials begin reporting (body composition, glucose/insulin, lipids, liver markers, inflammation)
The “mechanism markers” researchers care about (NNMT pathway / metabolites)
Safety signals & red flags worth watching (sleep, GI, mood, labs)
“Oral dosing” trends that are advertised/reported online in 2026 (not a recommendation)
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
#5A1MQ #5Amino1MQ #NNMT #NADPlus #MetabolicHealth #FatLossScience #Biohacking #Longevity #InsulinResistance #BloodSugar #FattyLiver #Sarcopenia #Mitochondria #HumanPerformance #TheHuman50Project

5-Amino-1MQ (5A1MQ) Explained (2026 Update): NNMT Inhibitor, NAD+ Salvage, Fat-Loss Data, Muscle Aging & Safety—No Hype

Is 5-Amino-1MQ (5A1MQ) the “fat-loss NAD+ compound” people claim—or is it just early-stage science getting overhyped? In this episode, JP (UltimateBiohacker10x / The Human 5.0 Project) breaks down what 5A1MQ actually is, how NNMT inhibition works, what the best animal data shows for obesity and fatty liver, what we’re seeing in aged muscle performance studies, and the most important part: what we still do NOT know in humans as of January 2026.
Key sources (for show notes):
NNMT inhibitor 5A1MQ cell/NAD+ findings (Neelakantan et al., 2017). PMC
DIO mouse obesity + fatty liver pathology + PK (Babula et al., 2024, Diabetes Obes Metab). PMC
Aged mice muscle function improvements with NNMT inhibition (Dimet-Wiley et al., 2024). PubMed+1
Human NNMT/MNA association with insulin resistance/T2D (Kannt et al., 2015). PMC
NNMT + NAD+ / aging review context (Li et al., 2024). PMC
Disclaimer: Educational content only. Not medical advice.
 
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
#5A1MQ #5Amino1MQ #NNMT #NAD #NADPlus #MetabolicHealth #FatLossScience #Longevity #Biohacking #Mitochondria #InsulinResistance #FattyLiver #Sarcopenia #HumanPerformance #Ultimatebiohacker10x

Why You Can Eat Bread in Europe but Not in the US (Gluten, Flour Processing, Additives Explained)

Welcome to The Human 5.0 Project with UltimateBioHacker10X.
Gluten has become the internet’s favorite scapegoat—but here’s the weird part: people who feel terrible after bread in the U.S. often report feeling totally fine eating bread in France, Italy, or Spain. Same person… different bread. So what’s really going on?
In this episode, we unpack:
Why US flour processing can be radically different (bleaching, enrichment, shelf-life priorities)
How long fermentation / sourdough-style methods may change digestibility
The role of additives & dough conditioners (and why ingredient lists matter)
Why some “gluten sensitivity” may actually be wheat components, FODMAPs, additives, or gut irritation—not gluten itself
Who should never “test it” casually (celiac disease + true wheat allergy)
Important: This podcast is for education only, not medical advice. If you suspect celiac disease or a wheat allergy, talk to a qualified clinician before changing your diet or “trialing” gluten.
 
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
If you want Part 2 (best bread choices + how to run a real elimination/reintroduction test without guesswork), Click here!
 
#Gluten #GlutenFree #Bread #GutHealth #Digestion #FoodScience #Sourdough #FODMAP #Wheat #Nutrition #Biohacking #Longevity #FoodAdditives #TheHuman50Project #UltimateBioHacker10X

SLU-PP-332 Side Effects (Part 2): Cardiomyopathy Rumors, Mouse Heart Data & What We Actually Know

Welcome back to The Human 5.0 Project with UltimateBioHacker10X
In Part 2 on SLU-PP-332, we skip the hype and talk side effects and the biggest online rumor: cardiomyopathy / heart risk. We break down what people commonly report anecdotally (like flushing, water retention, and fatigue when dosing too aggressively) and then zoom out to what preclinical mouse data can—and can’t—tell us about cardiac safety (histology, fibrosis signals, and functional echo-style outcomes).
Important: SLU-PP-332 is a preclinical research compound and is not approved for human use. This content is for education only and is not medical advice.
⏱️ In this episode:
Commonly reported “human chatter” side effects (anecdotes vs evidence)
The cardiomyopathy rumor: what preclinical endpoints look for
Heart markers and organ-system signals people reference (and limitations)
The real bottom line: what we know vs what we still don’t (humans)
 
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
#SLUPP332 #SLUPP332SideEffects #Peptides #Biohacking #Longevity #HumanPerformance #Mitochondria #ExerciseMimetic #Cardiomyopathy #HeartHealth #PreclinicalResearch #MouseStudies #WellnessScience #TheHuman50Project #UltimateBioHacker10X

GH Peptides Explained: Ipamorelin, CJC-1295, Tesamorelin, IGF-1 & Why Most People Use Them Wrong

Most people chase growth hormone peptides without understanding how the pituitary actually works — and that’s how you end up with hormone shutdown, stalled gains, or long-term damage.
In this episode of The Human 5.0 Project, Ultimate Biohacker 10X breaks down growth hormone–releasing peptides in plain English using a simple “phone call” analogy so you finally understand what these compounds actually do inside your body.
We cover:• How Ipamorelin, Sermorelin, Tesamorelin & CJC-1295 stimulate your pituitary• Why GH peptides are signals, not steroids• The difference between GH release vs IGF-1 muscle growth• How IGF-1 LR3 and PEG-MGF work downstream (and when they backfire)• Why overdosing or poor stacking turns your endocrine system into chaos• How prolactin, insulin, and receptor desensitization sabotage results• Which peptides stimulate other pituitary pathways (thyroid, libido, fertility)• Smart cycling, bloodwork timing, and safety rules most people ignore
If you’ve ever used — or considered using — growth hormone peptides for fat loss, muscle growth, recovery, or longevity, this episode explains why less is more and how to avoid long-term hormonal damage.
Download the FREE GH Peptide Cheat Sheet(dosing frameworks, cycling rules, bloodwork panels):
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
Comment below: Which peptide are you currently using — or considering?
Subscribe for weekly deep dives into peptides, hormones, longevity, and regenerative medicine.
Stay hacked. Stay curious. Stay hormonally intelligent.
 
#GrowthHormone#GHPeptides#Ipamorelin#CJC1295#Tesamorelin#Sermorelin#IGF1#PEGMGF#PeptideTherapy#HormoneOptimization#PituitaryHealth#EndocrineSystem#MuscleGrowth#FatLoss#Longevity#Biohacking#Human50Project#UltimateBiohacker10X

Pituitary Gland & HPA Axis Explained: Hormone Control, Stress, Growth & Healthspan

Your pituitary gland and HPA axis control nearly every hormonal signal in your body — from stress and metabolism to growth, reproduction, and long-term healthspan.
In this episode of The Human 5.0 Project, Ultimate Biohacker 10X delivers a clear, clinician-grade breakdown of how the pituitary gland actually works — without fluff or fear-mongering.
You’ll learn:• The difference between the posterior and anterior pituitary• How neural vs portal signaling controls hormone precision• ADH and oxytocin — water balance, bonding, and mood• Growth hormone, thyroid signaling, cortisol, and the HPA axis• How chronic stress rewires hormone output• Why pituitary dysfunction leads to fatigue, weight gain, anxiety, infertility, and autoimmune flares• What happens downstream when this system is optimized — or broken
This episode connects neuroscience, endocrinology, and real-world healthspan into one integrated framework.
 Download the full hormone signaling map & HPA axis PDF:
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
Subscribe for weekly deep dives into hormones, longevity, peptides, and performance.
Comment below: What hormone system do you want decoded next?
 
#PituitaryGland#HPAAxis#HormoneHealth#Endocrinology#StressHormones#Cortisol#GrowthHormone#ThyroidHealth#HormoneOptimization#Biohacking#Longevity#Healthspan#FunctionalMedicine#Human50Project#UltimateBiohacker10X

The Gut Microbiome Explained: How Your Gut Bacteria Control Health, Hormones & Immunity

The Gut Microbiome Explained: Why Your Health Starts in Your Intestines
 
Your gut microbiome isn’t just about digestion — it’s a living ecosystem of over 38 trillion microorganisms that regulate nearly every system in your body. From hormones and immunity to inflammation, metabolism, and even mood, your gut bacteria quietly shape your health from the inside out.
In this episode, we take a deep dive into the intestinal microbiome (oral microbiome coming next) and break down what the science actually shows.
Inside this episode:• When researchers discovered that gut bacteria outnumber human cells — and why that changed medicine• The real role of short-chain fatty acids (like butyrate) in gut lining repair and immune regulation• How dysbiosis contributes to autoimmune disease, Crohn’s, IBS, hormone imbalance, and chronic inflammation• The bacteria that matter most: Faecalibacterium, Akkermansia, and why losing them changes everything• Microbiome testing explained — GI-MAP, Viome, and what data actually matters• Why “leaky gut” starts with microbiome collapse and how to reverse it• Diet strategies that actually work: fiber diversity, resistant starch, fermented foods, and what to avoid
If you’re dealing with bloating, fatigue, hormone chaos, autoimmune symptoms, or unexplained inflammation — your gut ecosystem may be the missing link.
🎯 Download the free Gut Microbiome Reset PDFIncludes: testing breakdowns, food lists, supplement strategies, and gut-healing protocols
 
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
 
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
#GutMicrobiome #GutHealth #MicrobiomeScience #LeakyGut #IBD #AutoimmuneHealth #HormoneBalance #Butyrate #Akkermansia #Faecalibacterium #DigestiveHealth #Biohacking #Longevity #FunctionalMedicine #Human50Project #UltimateBiohacker10X

Methylation Explained: Do You Need Methylated Vitamins? Testing, Gut Health & Optimal Dosing

Methylation Explained: Testing, MTHFR, Methylated Vitamins & the Gut-Brain Connection
 
Ever feel like low energy, mood swings, or brain fog is holding you back? It might be methylation – the body's core biochemical process for gene regulation, detox, and neurotransmitter balance. In this 12-minute episode, we break down what methylation is, how to test for deficiency (MTHFR & homocysteine), if testing even matters, why methylated vitamins (like methylfolate & methyl-B12) outperform regular forms for many, and the gut's surprising role (bacteria in yogurt don't methylate, but microbiome influences epigenetics via B vitamins & metabolites).
Key takeaways:
• Methylation basics: Adding CH3 groups to DNA/proteins – essential for healthspan
• Deficiency signs & testing: MTHFR gene variants, homocysteine levels (goal <9 µmol/L)
• Methylated vitamins: Benefits for MTHFR folks (better absorption, no masking B12 deficiency)
• Gut connection: Bacteria produce B12 (methylation cofactor); microbiome shapes DNA methylation patterns
• Protocols: Supplements, diet tweaks, when to test
If you're optimizing for longevity, mood, or detox – this is your primer.
Download the free Methylation Cheat Sheet (testing guide, supplement doses, gut tips)
 
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
 
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
#Methylation #MTHFR #MethylatedVitamins #Methylfolate #MethylB12 #GutMicrobiome #Epigenetics #Homocysteine #DetoxPathways #MoodBoost #EnergyHack #Longevity #Biohacking #FunctionalMedicine #VitaminDeficiency #BVitamins #Human50Project #UltimateBiohacker10X

Top 10 Peptides of 2025: BPC-157, TB-500, Semaglutide & the Real Science Behind Modern Biohacking

 
The peptide revolution is here — and it’s changing how elite athletes, biohackers, and longevity clinicians approach healing, fat loss, and performance.
In this episode of The Human 5.0 Project, we break down the Top 10 Most Powerful Peptides of 2025, based on real-world use, clinical data, and years of hands-on experience — not hype.
You’ll learn what actually works, what’s overrated, and how advanced protocols are being used responsibly to optimize recovery, metabolism, and long-term health.
🔬 Covered in this episode:
• BPC-157 & TB-500 – The “Wolverine Stack” for tendon, ligament, and gut repair• Semaglutide (Ozempic) – Fat loss, insulin sensitivity, and metabolic reset• Tirzepatide – Next-gen GLP-1/GIP therapy with superior results• Retatrutide – Triple agonist changing the future of obesity treatment• MOTS-c – Mitochondrial performance and metabolic resilience• KPV – Gut inflammation and immune modulation• CJC-1295 + Ipamorelin – Growth hormone optimization without shutdown• IGF-1 LR3 – Muscle hypertrophy & recovery (with critical cautions)
We also cover:✔ Real-world dosing philosophies (not medical advice)✔ Why most people misuse peptides✔ What actually works vs internet hype✔ Safety considerations and long-term thinking
📘 Free Download:Get the Top 10 Peptides Cheat Sheet with dosing frameworks, cycle lengths, and stacking logic
 
For more longevity insights and protocols, check out biohackerdepot.com – your go-to resource for cutting-edge health optimization strategies.
 
And if you're sourcing research peptides , peptidesphere.com has a curated selection of peptides and bioregulators with detailed info on quality and applications.
 
 
#Peptides2025 #BPC157 #TB500 #WolverineStack #Semaglutide #Tirzepatide #Retatrutide #MOTSc #KPV #CJC1295 #Ipamorelin #IGF1LR3 #PeptideTherapy #Biohacking #RegenerativeMedicine #MuscleRecovery #GutHealing #WeightLossPeptides #LongevityPeptides #Human50Project #UltimateBiohacker10X